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文献和实验Glucuronidation of Fatty Acids and Prostaglandins by Human UDP-Glucuronosyltransferases
glucuronidation from both human liver micro-somes and human recombinant UDP-glucuronosyltransferase 2B7 (UGT2B7) are presented. Several unique methods for synthesis of FAs that are not commercially available are also described. Moreover, comprehensive methods
Phenotyping UDP-Glucuronosyltransferases (UGTs) Involved in Human Drug Metabolism: An Update
Glucuronidation, catalyzed by the UDP-glucuronosyltransferases (UGT), is a major drug clearance mechanism in humans and other mammalian species. UGT reaction phenotyping involves determining which of the 19 known human UGTs are primarily
Glycan Microarray Screening Assay for Glycosyltransferase Specificities
-based detection of product to assess enzyme activity. Here, we describe the application of both procedures to assess the specificity of a recombinant human α2-6 sialyltransferase. This technique is readily adaptable to studying other glycosyltransferases.
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