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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation
SUMO conjugation to protein substrates requires the concerted action of a dedicated E2 ubiquitin conjugation enzyme (Ubc9) and associated E3 ligases. Although Ubc9 can directly recognize and modify substrate lysine residues that occur
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
substrates to monitor DUB activity; and the purification of a recombinant human DUB, isopeptidase T, in E. coli using low-temperature expression as well as ion-exchange and affinity chromatography.
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