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文献和实验Functional Studies of Dual-Specificity Phosphatases
which are specific for phosphotyrosine, dual-specificity enzymes can dephosphorylate both phosphotyrosme and phosphoserine/phosphothreonine. All tyrosine phosphatases, including dual-specificity enzymes, are characterized by a conserved-sequence motif HCXXGXXRS/T
Dual- and Triple-Color Fluorospot
实验原理 ELISPOT has, due to its high sensitivity and simplicity, proven valuable for assessing antigen-specific T-cell responses, both with regard to specificity and magnitude. One limitation of the regular ELISPOT method
Purification of acidic phosphatase from mustard seedlings
of mustard (Sinapis alba ) and partially purifiy the enzyme by ammonium sulfate precipiation. Most important prerequisite for any enzyme isolation is an activity test. For this phosphatase, we take advantage of the broad substrate specificity and use
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