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文献和实验Preparation of Phytoplasma Membrane Recombinant Proteins
The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol for the heterologous expression
Expression of Recombinant Membrane-Type MMPs
” (1 ) there has been much interest in the production of recombinant protein to facilitate both structural and functional analyses. The proteolytic activity of the membrane-type-1 MMP (MT1-MMP) has been studied using recombinant material produced from expression in E. coli (2 ,3 ). These data
Construction of Recombinant Human Cytomegalovirus
The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral
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