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文献和实验Purification of Clostridium botulinum Type A Neurotoxin
Botulinum neurotoxins produced by strains of the spore-bearing bacterium Clostridium botulinum have long been known to cause a distinctive paralytic disease in humans and animals (1 ). In recent years, injection of crystalline botulinum
Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces
Clostridium botulinum is a diverse group of anaerobic spore-forming organisms that produce lethal botulinum neurotoxins (BoNT) during their growth. BoNTs cause a paralytic condition, botulism, to man and animals. The most common forms
Introduction of Recombinant DNA into Clostridium spp.
for such studies is a means of introducing recombinant DNA into individual viable cells, and the first reports of successful plasmid transformation involved use of protoplasts or L-forms (1 –4 ), or conjugal cointegrate transfer techniques
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