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文献和实验An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
acid residues in epitopes ( 12 ). These methods, however, involve extensive in vitro manipulations or are limited by available sites within the cloned sequence. A simple and inexpensive in vivo alternative ( 13 ), which is not constrained by restriction
13 Synthesis of Peptide Bioconjugates
and oligopeptides as epitopes with small molecular mass. The synthetic procedures outline three major strategies for the incorporation of multiple copies of uniformly oriented peptide epitopes. In the first example, chloroacetylated polypeptide is conjugated with SH
Evaluation of Autoimmunity to Transaldolase in Multiple Sclerosis
with multiple sclerosis are described. Recombinant transaldolase was produced in a prokaryotic expression vector for use in Western blot analysis of sera of these patients. Overlapping transaldolase peptides 15 amino acids (aa) long were synthesized
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