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文献和实验Adenoviral Gene Transfer into Isolated Pancreatic Islets
of insulin and glucagon production and secretion, as well as for gene therapy purposes for the treatment of diabetes. We describe here in detail a protocol for adenoviral gene transfer into isolated mouse islets of the pancreas. Effective gene transfer
PolyPhred Analysis Software for Mutation Detection from Fluorescence‐Based Sequence Data
Figure 7.16.3 (A ) Aligned reads window after changing view from “strand” to “alpha” in the Options list. This view facilitates review by placing forward and reverse reads next to one another. The red tag at the top indicates
event is mediated by cre recombinase and requires induction of the GAL1-10 promoter on galactose. Our strains grow poorly on galactose but give 80 to 100% popouts. The HA triple tag can be detected by mouse monoclonal antibodies 12CA5 (Boehringer
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