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文献和实验Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7
glycoproteins and particularly relevant for a first study of lot-to-lot comparison, or detection of unwanted glycans. In this chapter, we describe a lectin array-type method specifically designed for the study of recombinant therapeutic interleukin-7 (rhIL
Cloning of Human Telomeres in Saccharomyces cerevisiae
prophase nucleus. Isolation of telomeric sequences is therefore of interest with respect to unraveling telomere structure and the mechanisms underlying telomeric function. In addition, the isolation and characterization of all human telomeres is a necessary
Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae
is on expression of recombinant human integral MPs in a functional state at the plasma membrane of the yeast S. cerevisiae . Optimization of yeast culture and of MP preparations is detailed for two human receptors of the Hedgehog pathway: Patched and Smoothened.
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