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文献和实验Heavy Labeling of Recombinant Proteins
Because of the cost of isotopic chemicals and heterologous proteins to produce, an economical 15 N/13 C isotopic labeling method is critically needed. Four protocols have been tested for the expression of Ovine interferon-τ in Pichia pastoris
Bacterial Expression of Prolactin Family Proteins
Complementary DNAs of three recombinant proteins related to the prolactin family: ovine placental lactogen (oPL), ovine prolactin (oPRL), and rabbit soluble extracellular domain of prolactin receptor (rbPRLR-ECD) were subcloned by different
AAV-Mediated Gene Transfer to Skeletal Muscle
to nondividing cells such as muscle fibers or hepatocytes following in vivo transduction (1 –7 ). Recombinant AAV can be administered to skeletal muscle of experimental animals and, as recently documented in a Phase I clinical trial, to humans at high vector
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