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文献和实验Polyclonal Antibody Production
or cloned and expressed as a fusion protein with a larger protein. If it is a peptide or small molecule, a carrier-hapten conjugate must be made. Purify your antigen well to obtain antibodies with good specificity. Obtain at least two 6 week old New
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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