【注】:尿液、血清和血浆样品应在1/250-1/5000的范围内稀释后再进行检测,具体稀释倍数根据具体实验情况而定。 Table 1. Layout of Pyrophosphate standards and test samples in a solid black 96-well microplate.
BL
BL
TS
TS
PS1
PS1
…
…
PS2
PS2
PS3
PS3
PS4
PS4
PS5
PS5
PS6
PS6
PS7
PS7
PS = Pyrophosphate Standard (PS1 - PS7, 0.13 to 100 µM), BL = Blank Control, TS = Test Sample. Table 2. Reagent composition for each well.
Well
Volume
Reagent
PS1 - PS7
50 μL
Serial Dilutions (0.13 to 100 µM)
BL
50 μL
Assay Buffer
TS
50 μL
Test sample
对于96孔板,每孔加入50 μL PPi Sensor工作液,总测定体积为100 μL/孔;对于384孔板,每孔加入25 μL PPi工作液,总测定体积为50 μL/孔。