- 询价
- Signalway Antibody
- 56021
- 2025年09月22日
- WB IHC
- Recombinant Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human PMEL17 / GP100
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB IHC
- 靶点：
Uniprot：P40967
- 抗体英文名：
PMEL17 / GP100 Rabbit mAb
- 规格：
50ul/100ul
计算分子量:100kDa
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:WB:1:500~1:2000
IHC:1:50~1:200
图片:
Western blot analysis of PMEL17 / GP100 expression in human melanoma lysate.
,
Immunohistochemical analysis of paraffin-embedded human melanoma, using PMEL17 / GP100 Antibody.
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文献和实验1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
gives a good response. If experimental conditions require, a titration of cell densities (2-3x106 /ml to 105 /ml) should be performed for optimization. Add 100µl of the cell suspension to each well. For each condition, use triplicate wells.
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