Western Blot Validation in Mouse and Rat Tissue Lysates. Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, orb1274388, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
Western Blot Validation in HepG2 Cells. Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, orb1274388, at 1:500 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue. Immunohistochemistry of paraffin-embedded mouse kidney tissue using ACE2 antibody, orb1274388, at 1:100 dilution (40x lens).