剂型描述 Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.
内毒素 Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
纯度-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
明星产品 Recombinant Human Wnt3a V3蛋白(Cat#C22R)
(1)Wnt 3a V3产品活性及稳定性数据
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 71.45 ng/ml.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 71.45 ng/ml.
(2)类器官培养验证
小鼠肠道类器官。Mouse intestinal organoids were cultured with EGF (Cat#C029), Wnt3a V3 (Cat#C22R), Noggin (Cat#C028) and R-spondin 1 (Cat#CX83). After five days of culture, the organoids showed good morphology and germination rate. (Regularly tested)
小鼠结肠类器官。Mouse Colonic organoids were cultured with EGF (Cat#C029), Wnt3a V3 (Cat#C22R), Noggin (Cat#C028) , and R-spondin 1 (Cat#CX83). The organoids showed good morphology.
小鼠胃类器官。Mouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a V3 (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. (Regularly tested)