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7-Deaza-7-propargylamino-dATP tetralithium
MCE 国际站:7-Deaza-7-propargylamino-dATP tetralithium
产品活性:7-Deaza-7-propargylamino-dATP tetralithium 是脱氧腺苷三磷酸 (dATP) 的类似物,可用于基因测序。
研究领域:Others
作用靶点:Others
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文献和实验Bst-catalyzed radiolabeled DNA sequencing
. Each extension reaction contained 500-750ng of Biomek isolated single-stranded DNA, reaction buffer, nucleotide extension mix, oligonucleotide primer (typically M13 (-40) universal sequencing primer, see Appendix D), either [a-32-P]dATP or [a-35-S
Dideoxy Sequencing Reactions Using Taq Polymerase
(I) or 7-deazadGTP (II) in the reaction mixes. All reactions were performed as described in the text using an M 13mp 19 derived single-stranded templates labeled by incorporation of [(α-35 S] dATP. The reactions were analyzed on a 6% polyacrylamide-urea gel using
μl 20 mM dATP 80 μl 20 mM dCTP 240 μl 10 mM 7deaza-dGTP 80 μl 20 mM dTTP 1920 μl 5 mM ddATP 640 μl 50:1 TE buffer 3420 μl sddH2 O 6.4 ml 5X Taq "C" termination mix : 250 uM dATP, 62.5 uM dCTP, 375 uM c7dGTP, 250 uM dTTP and 0.75 mM
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