In Vitro: SG-094 (72 hours) shows antiproliferative effects against RIL175 cells, with an IC50 of 3.7 μM. SG-094 (10 μM) blocks PI(3,5)P2-elicited TPC2 currents on isolated endolysosomes from HEK293 cells expressing TPC2-EGFP. SG-094 (10 μM; pretreated for 1 h) significantly reduces VEGF-induced phosphorylation of eNOS, JNK, MAPK, and AKT without decreasing their total protein levels in HUVECs. SG-094 (1-5 μM; pretreated for 1 h) increases OCR/ECAR ratios of RIL175 WT cells after glucose starvation.
In Vivo: SG-094 (90 nmol/kg; every 2-3 days over a 10-day timescale) inhibits hepatocellular carcinoma (HCC) tumor growth in mice.