保存建议:大鼠SDF-1β(基质细胞衍生因子1Beta)ELISA试剂盒-Rat SDF-1β (Stromal Cell Derived Factor 1 Beta) ELISA KitBiomatik品牌厂家建议该大鼠SDF-1β(基质细胞衍生因子1Beta)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1β. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDF-1β. The concentration of Rat SDF-1β in samples can be calculated by comparing the OD of the samples to the standard curve.