保存建议:大鼠HNF1a(肝细胞核因子1Alpha)ELISA试剂盒-Rat HNF1a (Hepatocyte Nuclear Factor 1 Alpha) ELISA KitBiomatik品牌厂家建议该大鼠HNF1a(肝细胞核因子1Alpha)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HNF1a and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HNF1a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HNF1a. The concentration of Rat HNF1a in samples can be calculated by comparing the OD of the samples to the standard curve.