保存建议:小鼠15-LO(花生四烯酸15-脂氧合酶)ELISA试剂盒-Mouse 15-LO (Arachidonate 15-Lipoxygenase) ELISA KitBiomatik品牌厂家建议该小鼠15-LO(花生四烯酸15-脂氧合酶)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse 15-LO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse 15-LO. The concentration of Mouse 15-LO in samples can be calculated by comparing the OD of the samples to the standard curve.