保存建议:小鼠PTGS2/COX-2(前列腺素内过氧化物合酶2)ELISA试剂盒-Mouse PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA KitBiomatik品牌厂家建议该小鼠PTGS2/COX-2(前列腺素内过氧化物合酶2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PTGS2/COX-2. The concentration of Mouse PTGS2/COX-2 in samples can be calculated by comparing the OD of the samples to the standard curve.