保存建议:人MPO-Ab(抗髓过氧化物酶)ELISA试剂盒-Human MPO-Ab (Anti-Myeloperoxidase) ELISA KitBiomatik品牌厂家建议该人MPO-Ab(抗髓过氧化物酶)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human MPO-Ab. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for Human MPO-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the substrate solution is added to each well, only those wells that contain Human MPO-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MPO-Ab. The concentration of Human MPO-Ab in samples can be calculated by comparing the OD of the samples to the standard curve.