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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
艾美捷科技
- 应用:
说明书
- 适应物种:
说明书
- 标记物:
说明书
- 规格:
96T
关键词:人HSP-27/HSPB1(热休克蛋白27)ELISA试剂盒,Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit,Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit,人HSP-27/HSPB1(热休克蛋白27)ELISA试剂盒,Biomatik
产品名称:人HSP-27/HSPB1(热休克蛋白27)ELISA试剂盒-Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit
产品货号:EKE61078
产品规格:96T
背景资料:Biomatik是生物试剂一站式购物中心,提供29000+ELISA试剂盒,27000+抗体,17000+重组蛋白,280+生物试剂。
保存建议:人HSP-27/HSPB1(热休克蛋白27)ELISA试剂盒-Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA KitBiomatik品牌厂家建议该人HSP-27/HSPB1(热休克蛋白27)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-27/HSPB1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-27/HSPB1. The concentration of Human HSP-27/HSPB1 in samples can be calculated by comparing the OD of the samples to the standard curve.
点击:人HSP-27/HSPB1(热休克蛋白27)ELISA试剂盒-Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询艾美捷科技有限公司。更多Biomatik公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
Biomatik公司于2002年在加拿大成立,是生物试剂一站式购物中心,提供29000+ELISA试剂盒,27000+抗体,17000+重组蛋白,280+生物试剂。除了目录产品外,Biomatik还为全球的研究人员提供62,000多种定制产品,包括基因合成、多肽合成、蛋白及抗体生产的定制服务。
艾美捷科技有限公司,通过提供完整的产品和服务体系、便利的客户关系和供应链管理,为生命科学研究工作者提供整体打包方案。我们的企业的核心价值观:“学习、创新、合作、卓越"。作为一家具有尖端的技术实力、的经营管理水平和完善的市场销售体系的生物高科技企业,总部位于武汉光谷高新技术开发区,服务面向全国。艾美捷科技是集进口试剂、实验室耗材销售、技术服务与合约开发为一体的专业化高科技公司,为用户提供最前沿的专业资讯、完备的产品、整合的解决方案,及优质的物流服务。为了更好的服务客户,公司组建了一支经验丰富的研发团队-艾美捷生物技术中心,进入研发生产阶段,将更优质的产品推荐给国内生物领域的同仁们!
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文献和实验Isolation of Heat Shock Protein Complexes
Heat shock proteins (Hsp) are molecular chaperones with the capability to interact with a wide range of other proteins and are thus often found coupled with other heat shock and non-heat shock proteins. This can be an advantage to study
指将生物的整体、组织、细胞等从其生活的温度范围内急剧地从低温移向高温时,可显著地促进合成的一组蛋白质。例如将果蝇的幼虫或培养细胞从28℃移至 35℃时,则几乎大部分的蛋白质合成停止;与此相反,而休克蛋白的合成却反而被促进。这种促进作用主要是在转录 DNA的合成(转录)阶段产生的。同样的现象也见于哺乳类动物、培养细胞、原生动物、植物组织和细菌等。另外观察到,由休克以外的其他处理也会发生类似的现象。这种现象的生理意义尚不清楚,但推测是与生物的温度适应现象有关系。
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
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