保存建议:人PGF2α(前列腺素F2α)ELISA试剂盒-Human PGF2α (Prostaglandin F2α) ELISA KitBiomatik品牌厂家建议该人PGF2α(前列腺素F2α)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human PGF2α. During the reaction, Human PGF2α in the sample or standard competes with a fixed amount of Human PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.