背景资料:This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 18 (IL18). No significant cross-reactivity or interference between Mini Samples Interleukin 18 (IL18) and analogues was observed.
保存建议:人白介素18(IL18)ELISA试剂盒-Human Interleukin 18 (IL18) ELISA KitBiomatik品牌厂家建议该人白介素18(IL18)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosinase (TYR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosinase (TYR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosinase (TYR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosinase (TYR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.