背景资料:This assay has high sensitivity and excellent specificity for detection of Sulfatase 2 (SULF2). No significant cross-reactivity or interference between Sulfatase 2 (SULF2) and analogues was observed.
保存建议:小鼠硫酸酯酶2(SULF2)ELISA试剂盒-Mouse Sulfatase 2 (SULF2) ELISA KitBiomatik品牌厂家建议该小鼠硫酸酯酶2(SULF2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylenetetrahydrofolate Reductase (MTHFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylenetetrahydrofolate Reductase (MTHFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.