背景资料:This assay has high sensitivity and excellent specificity for detection of Proteinase 3 (PR3). No significant cross-reactivity or interference between Proteinase 3 (PR3) and analogues was observed.
保存建议:小鼠蛋白酶3(PR3)ELISA试剂盒-Mouse Proteinase 3 (PR3) ELISA KitBiomatik品牌厂家建议该小鼠蛋白酶3(PR3)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monokine Induced By Interferon Gamma (MIg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monokine Induced By Interferon Gamma (MIg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monokine Induced By Interferon Gamma (MIg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monokine Induced By Interferon Gamma (MIg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.