背景资料:This assay has high sensitivity and excellent specificity for detection of Pleiotrophin (PTN). No significant cross-reactivity or interference between Pleiotrophin (PTN) and analogues was observed.
保存建议:小鼠多效素(PTN)ELISA试剂盒-Mouse Pleiotrophin (PTN) ELISA KitBiomatik品牌厂家建议该小鼠多效素(PTN)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 2 (CYSLTR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteinyl Leukotriene Receptor 2 (CYSLTR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.