背景资料:This assay has high sensitivity and excellent specificity for detection of Mucin 1 (MUC1). No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.
保存建议:小鼠粘蛋白1(MUC1)ELISA试剂盒-Mouse Mucin 1 (MUC1) ELISA KitBiomatik品牌厂家建议该小鼠粘蛋白1(MUC1)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase 4 (PDK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase 4 (PDK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase 4 (PDK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase 4 (PDK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.