背景资料:This assay has high sensitivity and excellent specificity for detection of Lamin B2 (LMNB2). No significant cross-reactivity or interference between Lamin B2 (LMNB2) and analogues was observed.
保存建议:人层粘连蛋白B2(LMNB2)ELISA试剂盒-Human Lamin B2 (LMNB2) ELISA KitBiomatik品牌厂家建议该人层粘连蛋白B2(LMNB2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Rich Calcium Binding Protein (HRC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Rich Calcium Binding Protein (HRC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Rich Calcium Binding Protein (HRC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Rich Calcium Binding Protein (HRC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.