背景资料:This assay has high sensitivity and excellent specificity for detection of Cytokeratin 19 (CK19). No significant cross-reactivity or interference between Cytokeratin 19 (CK19) and analogues was observed.
保存建议:小鼠细胞角蛋白19(CK19)ELISA试剂盒-Mouse Cytokeratin 19 (CK19) ELISA KitBiomatik品牌厂家建议该小鼠细胞角蛋白19(CK19)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prekallikrein (PK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prekallikrein (PK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prekallikrein (PK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prekallikrein (PK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.