背景资料:This assay has high sensitivity and excellent specificity for detection of Hexokinase 1 (HK1). No significant cross-reactivity or interference between Hexokinase 1 (HK1) and analogues was observed.
保存建议:人己糖激酶1(HK1)ELISA试剂盒-Human Hexokinase 1 (HK1) ELISA KitBiomatik品牌厂家建议该人己糖激酶1(HK1)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Lipoxygenase (5-LO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Lipoxygenase (5-LO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Lipoxygenase (5-LO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Lipoxygenase (5-LO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.