背景资料:This assay has high sensitivity and excellent specificity for detection of Glutaminase 2 (GLS2). No significant cross-reactivity or interference between Glutaminase 2 (GLS2) and analogues was observed.
保存建议:小鼠谷氨酰胺酶2(GLS2)ELISA试剂盒-Mouse Glutaminase 2 (GLS2) ELISA KitBiomatik品牌厂家建议该小鼠谷氨酰胺酶2(GLS2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 6 (CST6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin 6 (CST6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 6 (CST6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin 6 (CST6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.