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Histone H2B (Acetyl K120) Rabb

it Polyclonal Antibody抗体,orb1152062,biorbyt
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  • ¥3770
  • biorbyt已认证
  • orb1152062
  • 英国
  • 2025年12月04日
  • WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Histone H2B (Acetyl K120) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Histone H2B (Acetyl K120) Rabbit Polyclonal Antibody

    • 靶点

      H2BC3

    • 浓度

      1mg/ml

    • 应用范围

      WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb1152062

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K120)

    • 规格

      25 ul

    产品描述:Histone H2B (Acetyl K120) Rabbit Polyclonal Antibody

    别名:H2BK120ac; Histone H2B(Acetyl Lys120); Acetyl Histone H2B(120); acetyl-Histone H2B(Lys120); Histone H2B/HIST1H2BD; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S; histone H2B type 1-B; H2B1B_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K120)

    预测反应性:Mouse, Rat

    分子量:14 kDa

    应用稀释比例:WB=1:500-2000

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P33778

    Note:For research use only.

    产品细节图片1
    Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

    产品细节图片2
    Cell type: HeLa + Serum starvation (12h) + SBA (5mM, 24h), Cross-linking conditions: No cross-linking, Amount of chromatin per IP: 5×106 cells, Amount of ab per IP: 4 ug, 12 ug, Beads type and amount per IP: 50 µl of Protein A/G MagBeads, Comment: The ChIP was performed with 1 µg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH Promoter, GAPDH CDS region, RPL30, FOXO3a-promoter and FOXO3a-downstream. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

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