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- 文献和实验
- 技术资料
- 英文名：
UltraRainbow Calibration Particles, 6 peaks, 8.0-12.9µm, 1E7/mL, 2mL
- 供应商：
上海博耀商贸有限公司
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文献和实验Yeast Cell Cycle by Flow Cytometry
Na citrate containing 2 µM Sytox Green, so that final concentration in the sample is 1 µM. (Optional) Just before processing the cells, sonicate for 45 s again leaving cells in the 5 ml Falcon tubes. Sonication prevents
Natural History Museum Protocol for EST sequencing from lamb
- (if the agarose gel in step 4 only shows a weak band, use less than 50µl of water to elute). Direct Purification Buffer: 50mM KCl (=0.37g/100ml) 10mM Tris-HCL (pH 8.8 at 25℃) (=0.12g/100ml) 1.5mM MgCl2 (=0.03g/100ml) 0.1% Triton X-100 (=100µl/100ml
stock of 25ng/µl calf thymus DNA was used.) 5) Hoechst stain (1mg/ml) was diluted in 25 ml of 1 x TNE (filtered: 10mM Tris, 1mM EDTA, 0.1M NaCl) to a final concentration of 100ng/ml. The solutions were kept in 25 ml sterile specimen bottles (Granier
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