Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
FC, IHC, WB
宿主:
Rabbit
适应物种:
Human, Mouse, Rat
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb865387
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
POLYCLONAL
保存条件:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
形态:
Lyophilized
亚型:
Rabbit IgG
免疫原:
A synthetic peptide corresponding to a sequence of human EIF4EBP1 (PMEASQSHLRNSPEDK).
Flow Cytometry analysis of A431 cells using anti-EIF4EBP1 antibody (orb865387). Overlay histogram showing A431 cells stained with orb865387 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4EBP1 Antibody (orb865387, 1 μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IHC analysis of EIF4EBP1 using anti-EIF4EBP1 antibody (orb865387). EIF4EBP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF4EBP1 Antibody (orb865387) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.
Western blot analysis of EIF4EBP1 using anti-EIF4EBP1 antibody (orb865387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4EBP1 antigen affinity purified polyclonal antibody (Catalog # orb865387) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for EIF4EBP1 at approximately 17 kDa. The expected band size for EIF4EBP1 is at 17 kDa.