Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
ELISA, FC, WB
宿主:
Rabbit
适应物种:
Human, Mouse
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb865498
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
POLYCLONAL
保存条件:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
形态:
Lyophilized
亚型:
Rabbit IgG
免疫原:
E.coli-derived human DAO recombinant protein (Position: C18-K338).
Flow Cytometry analysis of U937 cells using anti-DAO antibody (orb865498). Overlay histogram showing U937 cells stained with orb865498 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAO Antibody (orb865498, 1 μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of DAO using anti-DAO antibody (orb865498). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAO antigen affinity purified polyclonal antibody (Catalog # orb865498) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for DAO at approximately 39 kDa. The expected band size for DAO is at 39 kDa.