Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
ELISA, FC, WB
宿主:
Rabbit
适应物种:
Human
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb865649
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
POLYCLONAL
保存条件:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
形态:
Lyophilized
亚型:
Rabbit IgG
免疫原:
E.coli-derived human RFC/SLC19A1 recombinant protein (Position: V198-N231).
Flow Cytometry analysis of JK cells using anti-RFC/SLC19A1 antibody (orb865649). Overlay histogram showing JK cells stained with orb865649 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFC/SLC19A1 Antibody (orb865649, 1 μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of RFC/SLC19A1 using anti-RFC/SLC19A1 antibody (orb865649). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC/SLC19A1 antigen affinity purified polyclonal antibody (Catalog # orb865649) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for RFC/SLC19A1 at approximately 65 kDa. The expected band size for RFC/SLC19A1 is at 65 kDa.