Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
ICC, IF, WB
宿主:
Mouse
适应物种:
Human, Mouse, Rat
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb865663
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
MONOCLONAL
保存条件:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
形态:
Lyophilized
亚型:
Mouse IgG2b
免疫原:
E.coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).
IF analysis of HSD3B1 using anti-HSD3B1 antibody (orb865663). HSD3B1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-HSD3B1 Antibody (orb865663) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (orb865663). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSD3B1 antigen affinity purified monoclonal antibody (Catalog # orb865663) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 42 kDa. The expected band size for HSD3B1 is at 42 kDa.