Anti-EMC4 Polyclonal Antibody

产品简介

靶标	EMC4
别名	Cell proliferation-inducing gene 17 protein; EMC4; ER membrane protein complex subunit 4; HSPC184; PIG17; tmem85; TMM85_HUMAN; Transmembrane protein 85;
背景资料	Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Swiss Prot	Q5J8M3
Gene ID	51234
亚细胞定位	Cytoplasm
宿主	Rabbit
克隆类型	Polyclonal Antibody
亚型	IgG
预测交叉反应	Cow;Chimpanzee;Xenopus laevis;Xenopus tropicalis
蛋白分子量	15/17/20kDa
实际分子量	19kDa
稀释比例	WB 1:1000-3000. IHC 1:50-200.
免疫原	A synthetic peptide of human EMC4
Public Immunogen Range	20-70/183aa
纯化方法	Affinity purification
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存	Store at -20℃. Avoid freeze / thaw cycles.
交叉反应	Human Mouse Rat
应用	WB IHC
规格	50ul 100ul
单位	支

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Immunohistochemistry of paraffin-embedded human stomach cancer with EMC4 antibody diluted at 1:100.

Western blot analysis with EMC4 antibody diluted at 1:2000;Lane: 293T,HepG2,A549,HeLa,SH-SY5Y,THP-1,U937,HepG2,Jurkat.