This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated.
Swiss Prot
Q96MS0
亚细胞定位
Cell membrane Cytoplasm
宿主
Rabbit
克隆类型
Polyclonal Antibody
亚型
IgG
蛋白分子量
111/148kDa
实际分子量
148kDa
稀释比例
WB 1:1000-3000.
免疫原
Recombinant protein of human ROBO3
Public Immunogen Range
1-148aa
纯化方法
Octanoic acid-ammonium sulfate precipitation
储存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存
Store at -20℃. Avoid freeze / thaw cycles.
交叉反应
Human;Mouse
应用
WB
规格
50ul 100ul
单位
支
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示:
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with ROBO3 antibody diluted at 1:2000;Lane: Rat liver.