Anti-PTRH2 Polyclonal Antibody

产品简介

单位	支
英文名称	Anti-PTRH2 Polyclonal Antibody
别名	BIT1;CFAP37;CGI-147;IMNEPD;PTH;PTH 2;PTH2
宿主	Rabbit
稀释比例	WB 1:500-2000. IHC 1:50-200. IF 1:10-100.
Gene ID	51651
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q9Y3E5
蛋白分子量	19kDa
储存液	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
亚细胞定位	Mitochondrion
免疫原	Recombinant protein of human PTRH2
亚型	IgG
性状	液体
纯化方法	Affinity purification
背景资料	The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms.
Public Immunogen Range	Recombinant protein of human PTRH2
克隆类型	Polyclonal Antibody
交叉反应	Human Rat Mouse
应用	WB IF IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with PTRH2 antibody diluted at 1:1000

Immunohistochemistry of paraffin-embedded rat brain tissue using PTRH2 antibody at dilution of 1:100 (x40 lens).

Immunofluorescence analysis of MCF-7 cell using PTRH2 antibody. Blue: DAPI for nuclear staining.