Anti-BCAR3 Polyclonal Antibody

Anti-BCAR3 Polyclonal Antibody


产品简介

靶标	BCAR3
别名	BCAR 3; BCAR3; BCAR3_HUMAN; Breast cancer anti estrogen resistance 3; Breast cancer anti estrogen resistance protein 3; Breast cancer anti-estrogen resistance protein 3; Breast cancer antiestrogen resistance 3
宿主	Rabbit
稀释比例	WB 1:1000-3000.;IHC 1:50-200.
Gene ID	8412
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	O75815
蛋白分子量	56/82/93kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm
免疫原	A synthetic peptide of human BCAR3
亚型	IgG
纯化方法	Affinity purification
背景资料	Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers (PubMed:24216110). Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis (PubMed:24216110). Promotes insulin-mediated membrane ruffling (By similarity). In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1 (PubMed:19086031). Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity (By similarity). May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction (By similarity). Regulates EGF-induced DNA synthesis (PubMed:18722344). Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling (By similarity). Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA (By similarity). Positively regulates integrin-induced tyrosine phosphorylation of BCAR1 (By similarity). Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS (By similarity). However, in a contrasting study, lacks GEF activity towards RAP1 (PubMed:22081014).
Public Immunogen Range	430-480/825aa
克隆类型	Polyclonal Antibody
交叉反应	Human
应用	WB;IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Western blot analysis with BCAR3 antibody diluted at 1:2000;Lane:HepG2,T-47d,HeLa,U-251

Immunohistochemistry of paraffin-embedded Human colorectal cancer with BCAR3 antibody diluted at 1:100.

Immunohistochemistry of paraffin-embedded Human endometrial cancer with BCAR3 antibody diluted at 1:100.