Anti-PRKAG1 Polyclonal Antibod

产品简介

单位	支
英文名称	Anti-PRKAG1 Polyclonal Antibody
别名	AMPKG
宿主	Rabbit
稀释比例	WB 1:500-2000. IHC 1:50-200. IF 1:50-100.
Gene ID	5571
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P54619
蛋白分子量	38kDa
储存液	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
亚细胞定位	Cytosol Nucleus
免疫原	Recombinant protein of human PRKAG1
亚型	IgG
性状	液体
纯化方法	Affinity purification
背景资料	The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Public Immunogen Range	Recombinant protein of human PRKAG1
克隆类型	Polyclonal Antibody
交叉反应	Human Rat Mouse
应用	WB IF IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with PRKAG1 antibody diluted at 1:1000

Immunohistochemistry of paraffin-embedded human stomach using PRKAG1 antibody at dilution of 1:100 (x40 lens).

Immunofluorescence analysis of MCF7 cell using PRKAG1 antibody. Blue: DAPI for nuclear staining.