Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs), a post-transcriptional modification necessary for their translation. Acts at position 390 in COXI mt-mRNA and at position 697-699 in mitochondrial COXIII mt-mRNA (PubMed:27974379). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and may play a role in mitochondrial ribosome biogenesis.
Swiss Prot
Q6P087
Gene ID
285367
亚细胞定位
Cytoplasm
宿主
Rabbit
克隆类型
Polyclonal Antibody
亚型
IgG
预测交叉反应
Bovine
蛋白分子量
15/37/38kDa
稀释比例
WB 1:1000-3000.;IHC 1:100-300.
免疫原
Recombinant protein of human RPUSD3
Public Immunogen Range
1-344aa
纯化方法
Affinity purification
储存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.4.
保存
Store at -20°C. Avoid freeze / thaw cycles.
交叉反应
Human Mouse Predicted:Bovine
应用
WB;IHC
规格
50ul 100ul
单位
支
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示:
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with RPUSD3 antibody diluted at 1:2000;Lane: 293T,PC-3M-IE8,Mouse brain.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with RPUSD3 antibody diluted at 1:200.