EST1-like protein A;Smg-6 homolog;hSmg5/7a;C17orf31;EST1A;KIAA0732
背景资料
Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PubMed:19179534). May have a general role in telomere regulation (PubMed:12676087, PubMed:12699629). Promotes in vitro the ability of TERT to elongate telomeres (PubMed:12676087, PubMed:12699629). Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization (PubMed:12676087, PubMed:12699629). Binds to the single-stranded 5'-(GTGTGG)4GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER) (PubMed:12676087, PubMed:12699629).Plays a role in nonsense-mediated mRNA decay (PubMed:18974281, PubMed:19060897, PubMed:20930030, PubMed:17053788). Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (PubMed:18974281, PubMed:19060897, PubMed:20930030, PubMed:17053788). Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA (PubMed:18974281, PubMed:19060897, PubMed:20930030, PubMed:17053788).
Swiss Prot
Q86US8
Gene ID
23293
亚细胞定位
Cytoplasm Nucleus
宿主
Rabbit
克隆类型
Polyclonal Antibody
亚型
IgG
预测交叉反应
Orangutan
蛋白分子量
37/57/160kDa
稀释比例
IHC 1:100-300.
免疫原
Recombinant protein of human SMG6
Public Immunogen Range
1-350aa
纯化方法
Affinity purification
储存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存
Store at -20°C. Avoid freeze / thaw cycles.
应用
IHC
规格
50ul 100ul
单位
支
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示:
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Immunohistochemistry of paraffin-embedded Human colorectal cancer with SMG6 antibody diluted at 1:200.