Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
蛋白分子量
48/56kDa
亚细胞定位
Cell membrane Cytoplasm Nucleus
免疫原
A synthetic peptide of human AKT1
亚型
IgG
性状
液体
纯化方法
Affinity purification
背景资料
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Public Immunogen Range
Recombinant protein of human AKT1
克隆类型
Polyclonal Antibody
交叉反应
Human Rat Mouse
应用
WB IF IHC
规格
50ul 100ul
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示:
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with AKT1 antibody diluted at 1:1000
Immunohistochemistry of paraffin-embedded human prostate tissue using AKT1 antibody at dilution of 1:100 (x40 lens).