serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
规格:
48 Test
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tartrate Resistant Acid Phosphatase 5b(TRACP-5b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tartrate Resistant Acid Phosphatase 5b(TRACP-5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tartrate Resistant Acid Phosphatase 5b(TRACP-5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tartrate Resistant Acid Phosphatase 5b(TRACP-5b) in the samples is then determined by comparing the OD of the samples to the standard curve.
产品名:Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit 酶联免疫试剂盒
货号:orb1146716
产品中文名:Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit 酶联免疫试剂盒
产品详情链接:https://www.biorbyt.com.cn/mouse-tracp-5b-tartrate-resist-orb1146716.html 武汉博欧特生物科技有限公司(www.biorbyt.com.cn )为英国品牌Biorbyt(www.biorbyt.com )中国区总代理。负责biorbyt品牌产品在中国市场的推广、销售及售后服务。为您提供各种biorbyt原装进口产品,主推四大类: