Mouse GATA Binding Protein 3 (

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GATA Binding Protein 3(GATA3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GATA Binding Protein 3(GATA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GATA Binding Protein 3(GATA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GATA Binding Protein 3(GATA3) in the samples is then determined by comparing the OD of the samples to the standard curve.

产品别名：HDR, Trans-acting T-cell-specific transcription factor GATA-3

应用笔记：standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GATA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GATA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GATA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GATA3 in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:P23772

Note：For research use only.