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- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
31.25-2000 pg/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
12 pg/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥3861.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥5499.0 |
描述: Sandwich Human sICAM1 ELISA Kit
别名: CD54 ELISA Kit, BB2 ELISA Kit, P3.58 ELISA Kit, Human Rhinovirus Receptor ELISA Kit, Major group rhinovirus receptor ELISA Kit
浓度: 2000 pg/mL
应用说明: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sICAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sICAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sICAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sICAM1 in the samples is then determined by comparing the OD of the samples to the standard curve
检测时间: 3.5h
研究领域: Cell Biology
备注: For research use only.

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文献和实验【资源】[共享]Adhesion Protein Protocols——(很有用的)
Materials 2.1— Construction of Adhesion Molecule—Ig Chimeras 2.2— Electroporation of Cells 2.3— Harvest and Culture of Transfectant Clones 2.4— Selection (ELISA) and Subcloning 2.5— Purification of Chimeric Proteins 3— Methods 3.1—
组。 3. 可溶性细胞间粘附分子 5 细胞间粘附分子 5(Intercellular adhesion molecule-5,ICAM5)是一种中枢神经系统来源的抗炎蛋白,它是一种神经元糖蛋白,在脑中表达,也可存在于血液中,经由T细胞移动性和趋化作用的抑制而作为抗炎蛋白发挥功能。 可溶性细胞间粘附分子(soluble Intercellular adhesion molecule-5,sICAM5)可以作为帮助我们诊断癫痫的生物标志物。在癫痫患者血液中,sICAM-5浓度降低,而促进炎症的白细胞
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
above. The epitope of some antibodies is not yet identified, an example being the rat type I cell-specific antibody, VIIIB2. Type I cells are also reported to express carbopeptidase membrane-bound enzyme (CP-M), intercellular adhesion molecule-1 (ICAM-1), &beta
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