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General Heparin (Hep) ELISA Ki

t试剂盒,orb782056,biorbyt
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  • ¥4290
  • biorbyt已认证
  • 15.63-1000 ng/mL
  • 4.63 ng/mL
  • orb782056
  • 英国
  • 2026年01月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      15.63-1000 ng/mL

    • 检测方法

      Competitive

    • 应用

      ELISA

    • 适应物种

      All

    • 样本

      serum, plasma and other biological fluids

    • 灵敏度

      4.63 ng/mL

    • 规格

      48 T

    产品描述:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Heparin(Hep) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparin(Hep).

    产品别名:Unfractionated heparin ELISA Kit

    应用笔记:standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hep protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hep. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hep in the samples is then determined by comparing the OD of the samples to the standard curve

    实验时长:2h

    Note:For research use only.

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    图标文献和实验
    相关实验
    • secondary antibody review -- data from 99 publications

      western blot   test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT),   17 goat IgG   FITC flow cytometry 1:250   Jackson ImmunoResearch Laboratories 17 goat     AP ELISA 1:1000     18       HRP western blot     Cell

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